ESTIMATING THE RATE OF PLASMID TRANSFER - AN END-POINT METHOD

被引:130
作者
SIMONSEN, L [1 ]
GORDON, DM [1 ]
STEWART, FM [1 ]
LEVIN, BR [1 ]
机构
[1] BROWN UNIV,DEPT MATH,PROVIDENCE,RI 02912
来源
JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷
关键词
D O I
10.1099/00221287-136-11-2319
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We describe a method for determining the rate parameter of conjugative plasmid transfer that is based on single estimates of donor, recipient and transconjugant densities and the growth rate in exponential phase of the mating culture. The formula for estimating the plasmid transfer rate, γ, was derived from a mathematical model describing cell growth and plasmid transfer in batch culture. Computer simulations were used to explore the sensitivity of this method to the realities of bacterial life, such as growth rate differences, plasmid segregation and transitory derepression of pilus synthesis. As predicted by the theory, mating experiments with the plasmid R1 in Escherichia coli K12 demonstrated that the estimate γ is unaffected by cell density, donor:recipient ratio and mating time. Unlike previous techniques, our method allows us to investigate the effect of environmental factors on plasmid transfer rates when these factors also influence population growth rates. to illustrate this, we examined the effect of temperature on the rate of plasmid transfer.
引用
收藏
页码:2319 / 2325
页数:7
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