A PRODUCT OF ACTIVATED HUMAN GRANULOCYTES STIMULATES PROSTAGLANDIN-E2 SYNTHESIS IN HUMAN AMNION CELLS

被引:2
作者
BRY, K [1 ]
HALLMAN, M [1 ]
机构
[1] UNIV CALIF IRVINE, DIV NEONATOL, IRVINE, CA 92717 USA
来源
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1991年 / 43卷 / 01期
关键词
D O I
10.1016/0952-3278(91)90130-W
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cell-free supernatant from formylmethionyl-leucyl-phenylalanine (fMLP)-activated granulocytes causes a time- and concentration-dependent stimulation of prostaglandin E2 (PGE2) production in amnion cells. PGE2 concentration in the culture medium after 36 h treatment with granulocyte supernatant (from 40 x 10(6) granulocytes/ml of amnion cell medium), 1.49 +/- 0.71 pg/ng DNA (n = 13), was significantly higher (p = 0.0015) than in control cells (0.33 +/- 0.23 pg/ng DNA, n = 13). Indomethacin abolished this stimulation. Granulocyte supernatant and human epidermal growth factor (hEGF) had an additive effect on amnion cell PGE2 production. Catalase, superoxide dismutase (SOD), protease inhibitors or the platelet-activating factor (PAF) antagonist L-659,989 had no effect. Actinomycin D, cycloheximide and mepacrine reduced the PGE2 production. The phospholipase A2 activity present in granulocyte supernatants was resistant to heating, whereas heating decreased their PGe2-stimulating activity by 92%. Exogenous phospholipase A2 had no effect on PGE2 synthesis. The granulocyte product could be precipitated with ammonium sulphate. On gel filtration of supernatant, two peaks of PGE2-synthesis stimulating activity were obtained (molecular weights 12 000 and 60 000). This data serve to explain the association of chorioamnionitis with preterm labor: activated granulocytes release a protein(s) that induces prostaglandin production in amnion cells, and thus promote labor.
引用
收藏
页码:35 / 42
页数:8
相关论文
共 34 条
  • [1] EXTRACELLULAR-MATRIX COMPONENTS SYNTHESIZED BY HUMAN AMNIOTIC EPITHELIAL-CELLS IN CULTURE
    ALITALO, K
    KURKINEN, M
    VAHERI, A
    KRIEG, T
    TIMPL, R
    [J]. CELL, 1980, 19 (04) : 1053 - 1062
  • [2] BALSINDE J, 1988, J BIOL CHEM, V263, P1929
  • [3] BEJAR R, 1981, OBSTET GYNECOL, V57, P479
  • [4] BILLAH MM, 1985, PROSTAGLANDINS, V30, P841
  • [5] BLEASDALE JE, 1985, REV PERINATAL MED, V5, P151
  • [6] BOYUM A, 1984, METHOD ENZYMOL, V108, P88
  • [7] CASEY ML, 1986, SEMIN PERINATOL, V10, P270
  • [8] EPIDERMAL GROWTH FACTOR-STIMULATED PROSTAGLANDIN-E2 PRODUCTION IN HUMAN AMNION CELLS - SPECIFICITY AND NONESTERIFIED ARACHIDONIC-ACID DEPENDENCY
    CASEY, ML
    MITCHELL, MD
    MACDONALD, PC
    [J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1987, 53 (03) : 169 - 176
  • [9] CASEY ML, 1988, J BIOL CHEM, V263, P7846
  • [10] Dewald B, 1986, Methods Enzymol, V132, P267