INFLUENCE OF POLYMORPHONUCLEAR GRANULOCYTES ON THE ZONA-FREE HAMSTER OOCYTE ASSAY

被引：18

作者：

VOGELPOEL, FR ^{[1
]}

VANKOOIJ, RJ ^{[1
]}

TEVELDE, ER ^{[1
]}

VERHOEF, J ^{[1
]}

机构：

[1] ACAD HOSP UTRECHT,DEPT CLIN BACTERIOL,3584 CX UTRECHT,NETHERLANDS

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 08期

关键词：

HUMAN SPERMATOZOA; OXYGEN RADICALS; POLYMORPHONUCLEAR GRANULOCYTES; ZONA-FREE HAMSTER OOCYTE ASSAY;

D O I：

10.1093/oxfordjournals.humrep.a137493

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

To gain more insight into a possible detrimental influence of polymorphonuclear granulocytes (PMN) on fertility, PMN were added to spermatozoa and oocytes in the hamster oocyte assay. Addition of 2.5 x 10(6)/ml PMN to the sperm suspension at the start of the first incubation period (capacitation period) resulted in a significant decrease (P < 0.03) in the number of decondensed sperm-heads per hamster oocyte (the decondensation index). When a concentration of 0.5 x 10(6)/ml PMN was used, no decrease was found. Stimulation of PMN by phorbol myristate acetate (PMA) did not enhance the inhibitory action of PMN. After incubation of oocytes with PMN for 1 h, an almost total inhibition of decondensation was observed. A significant inhibition could also be obtained when unstimulated PMN (2.5 x 10(6)) were added at the start often 3 h coincubation period of spermatozoa and oocytes (P < 0.004). The inhibition by PMN decreased to a non-significant level when the hydroxyl radical scavenger thiourea was present. The possible mechanisms by which PMN affect the results of the hamster oocyte assay and the possible implications of the presence of PMN in the genital tract are discussed.

引用

页码：1104 / 1107

页数：4

共 9 条

[1]

Aitken R.J., Elton R.A., Quantitative analysis of sperm-egg interaction in the zona-free hamster egg penetration test, Int, J, Androl., 2, 6, pp. 14-30, (1986)

[2]

Aust S.D., White B.C., The role of iron in lipid peroxidation, Free Radicals, Cell Damage and Disease, pp. 15-27, (1986)

[3]

Austin C.R., Short R.V., The Blood Testis Barrier, in Germ Cells and Fertilization, pp. 81-82, (1982)

[4]

Baker H., Straffon W., Murphy G., Davidson A., Burger H.G., De Kretser D.M., Prostatitis and male infertility: A pilot study, Possible increase in sperm motility with antibacterial chemotherapy, Int, J, Androl, 2, pp. 193-201, (1979)

[5]

Biggers J.D., Whitten W.K., Whittingham D.G., The culture of mouse embryos in vitro, Methods in Mammalian Embryology, pp. 86-116, (1971)

[6]

Chacho K.J., Resen P.J., Scommegna A., The effect of peritoneal macrophage incubates on the spermatozoa assay, Fertil, Sterii, 4, pp. 694-699, (1987)

[7]

Comhaire F., Verschraegen G., Vermeuien L., Diagnosis of accessory gland infection and its possible role in male infertility, Int, J, Androl, 3, pp. 32-45, (1980)

[8]

Fowikes D.M., Dooher G.B., O'Leary W.M., Evidence by scanning microscopy for an association between spermatozoa and T-mycoplasma in men of infertile marriage, Fertil, Sterii, 26, (1975)

[9]

Hogarth P.J., Autoimmunity in the Male, in Immunological Aspects of Mammalian Reproduction, pp. 39-45, (1982)

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