INVESTIGATION OF SOME ANTIMICROBIAL PROCEDURES ON THE INVITRO DEVELOPMENT OF EARLY MURINE EMBRYOS AIMED TOWARD DEVELOPING METHODS FOR THE DISINFECTION OF MAMMALIAN EMBRYOS PRIOR TO TRANSFER

被引：11

作者：

BIELANSKI, A

HARE, WCD

机构：

[1] Animal Diseases Research Institute, Agriculture Canada, Nepean, K2H 8P9, Ontario

来源：

JOURNAL OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER | 1991年 / 8卷 / 01期

关键词：

EMBRYOS; MICROORGANISMS; PHOTOSENSITIZERS; ENZYMES; PH;

D O I：

10.1007/BF01131587

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Eight-cell, zona pellucida-intact mouse embryos were exposed to the following substances or procedures that have been reported to have germicidal effects to determine if he embryos would survive and develop under in vitro conditions: the photosensitive substances hematoporphyrin, hematoporphyrin derivative, 8-methoxypsoralen, 4,5',8-trimethylpsoralen, and thiopyronine; the enzymes lipase (0.5%), phospholipase C (2 U/ml), chymotrypsin (0.5%), and trypsin (0.5%); pH 5.0; and helium/neon laser light, visible light, ultraviolet A light, and ultraviolet C light. Under the conditions used, embryos were not adversely affected by hematoporphyrin and/or helium/neon laser light; methoxypsoralen and/or ultraviolet A light; lipase; trypsin; pH 5.0 for 20 min; and visible light. Variable results were obtained from hematoporphyrin derivative with lase light. Thiopyronine, trimethylpsoralen in combination with ultraviolet A light, and ultraviolet C light killed embryos, and chymotrypsin and phospholipase C were harmful at 10- and 15-min exposure times, respectively.

引用

页码：24 / 32

页数：9

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