AN ENZYME-IMMUNOASSAY FOR HUMAN DEFENSINS

被引：62

作者：

PANYUTICH, AV

VOITENOK, NN

LEHRER, RI

GANZ, T

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,CHS 37-055,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,WILL ROGERS PULM RES LAB,LOS ANGELES,CA 90024

[3] BYELORUSSIAN INST HEMATOL & BLOOD TRANSFUS,MINSK,BELORUSSIA,USSR

[4] MOSCOW EXPTL HEMATOL & BIOTECHNOL INST,MOSCOW,USSR

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 141卷 / 02期

关键词：

DEFENSIN; NEUTROPHIL HUMAN; ENZYME IMMUNOASSAY; MONOCLONAL ANTIBODY; HOST DEFENSE; INFLAMMATION;

D O I：

10.1016/0022-1759(91)90141-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed and optimized an enzyme immunoassay for human neutrophil defensins, cationic cysteine-rich peptides that participate in host defense and inflammation. The assay utilizes a sandwich design with a monoclonal capture antibody and a biotinylated monoclonal detecting antibody. Cetrimonium bromide is employed to obviate non-specific binding of defensins to surfaces. The assay has a sensitivity of 0.04-0.05 ng/ml and a working range of 0.05-10 ng/ml.

引用

页码：149 / 155

页数：7

共 10 条

[1]

BORTH W, 1989, J BIOL CHEM, V264, P5818

[2] DEFENSINS - NATURAL PEPTIDE ANTIBIOTICS OF HUMAN-NEUTROPHILS [J].