THE CDNA CLONING, NUCLEOTIDE-SEQUENCE AND EXPRESSION OF AN INTRACELLULAR PROTEIN TYROSINE PHOSPHATASE FROM MOUSE TESTIS

被引：21

作者：

MIYASAKA, H ^{[1
]}

LI, SSL ^{[1
]}

机构：

[1] NIEHS,GENET LAB,RES TRIANGLE PK,NC 27709

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 185卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91700-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The PTP-1 cDNA encoding an intracellular protein tyrosine phosphatase (PTPase) was isolated and sequenced from a mouse testis cDNA library. This PTP-1 cDNA was found to contain an open reading frame of 1,296 nucleotides as well as 5′ (83 nucleotides) and 3′ (289 nucleotides) non-coding regions. The deduced sequence of 432 amino acids of mouse PTPase-1 exhibited 93% and 83% identity to that of rat PTPase-1 and human PTPase-1B, respectvely. Thus, this PTP-1 is a mouse homologue of human PTP-1B and rat PTP-1. Northern blot analysis indicated that PTP-1 mRNAs were most abundant in testis, and were detected in sizes of 4.4Kb, 2.4Kb and 2.2Kb, 2.0Kb. The PTP-1 transcripts of 4.4Kb and 2.0Kb, but not 2.4Kb and 2.2Kb, were also present in kidney, spleen, muscle, liver, heart and brain. Genomic blot analysis showed that a single copy of the PTP-1 gene is contained in the mouse genome and that introns are present in mammalian PTP-1 genes. © 1992.

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页码：818 / 825

页数：8

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