DEVELOPMENT OF A DEXTRAN KIT FOR LABELING WITH TC-99M AND ITS EVALUATION FOR LYMPHOSCINTIGRAPHY

被引：26

作者：

DASS, RS ^{[1
]}

SINGH, AK ^{[1
]}

CHAUHAN, UPS ^{[1
]}

机构：

[1] INMAS,DEPT RADIOPHARMACEUT,LUCKNOW 110054,DELHI,INDIA

来源：

NUCLEAR MEDICINE AND BIOLOGY | 1993年 / 20卷 / 05期

关键词：

D O I：

10.1016/0969-8051(93)90041-R

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

A cold dextran (molecular weight 60,000-90,000) kit has been developed by a modified procedure to produce instant preparation of Tc-99m dextran suitable for lymphoscintigraphy. The preparation was subjected to various quality control measures. Effect of pH and amount of stannous chloride as a reducing agent on the labelling efficiency using ITLC were studied. The labelled complex was separated from both the reduced pertechnetate and pertechnetate and quantified. In a series of experiments hydrolysed/reduced Tc-99m was found to be less than 3.0%, whereas pertechnetate was approx. 1.0%. Biokinetics of the agent in mice and blood clearance and rate of disappearance from the site of intradermal injection of the agent in rabbits were studied. The tagged agent for imaging of the lymphatic system activated by administering Freund's complete adjuvant (FCA)/E. coli in rabbits exhibited its suitability for lymphoscintigraphy.

引用

页码：701 / 706

页数：6

共 12 条

[1] QUANTITATIVE LYMPHOSCINTIGRAPHY FOR DETECTION OF METASTASES TO INTERNAL MAMMARY LYMPH-NODES - BIOKINETICS OF SULFUR-TC-99M COLLOID UPTAKE AND CORRELATION WITH MICROSCOPY [J].