A NEW MATRIX FOR AFFINITY-CHROMATOGRAPHY AND ITS APPLICATION IN THE SEPARATION OF A HUMAN MONOCLONAL-ANTIBODY

被引：1

作者：

GUO, W ^{[1
]}

SHANG, ZH ^{[1
]}

YU, YN ^{[1
]}

ZHOU, LM ^{[1
]}

机构：

[1] ACAD SINICA,DALIAN INST CHEM PHYS,POB 100,DALIAN 116012,PEOPLES R CHINA

来源：

BIOMEDICAL CHROMATOGRAPHY | 1994年 / 8卷 / 03期

关键词：

D O I：

10.1002/bmc.1130080310

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the preparation of a p-aminobenzene sulphonyl ethyl containing crosslinked Sepharose 4B (ABSE-Sepharose 4B-CL) is described. trypsin, bovine serum albumin (BSA) and concanavalin A (Con A) were immobilized onto this matrix by diazotization. Conditions for the coupling reaction were investigated. The activity of immobilized trypsin reached 1.4 X 10(4) U/g, and 25 mg BSA can be coupled onto 1 g ABSE-Sepharose 4B-CL under the optimal conditions. An affinity medium with immobilized Con A as ligand was prepared by this method, and was used in the separation of a human monoclonal antibody.

引用

页码：142 / 144

页数：3

共 6 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

Chaiken IM, 1987, ANAL AFFINITY CHROMA

[3] PREPARATION AND PROPERTIES OF 2 NEW CHROMOGENIC SUBSTRATES OF TRYPSIN [J].

ERLANGER, BF ;

COHEN, W ;

KOKOWSKY, N .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1961, 95 (02) :271-&

[4] AGAR DERIVATIVES FOR CHROMATOGRAPHY, ELECTROPHORESIS AND GEL-BOUND ENZYMES .1. DESULPHATED AND REDUCED CROSS-LINKED AGAR AND AGAROSE IN SPHERICAL BEAD FORM [J].

PORATH, J ;

JANSON, JC ;

LAAS, T .

JOURNAL OF CHROMATOGRAPHY, 1971, 60 (02) :167-&

[5]

TURKOVA, 1978, J CHROMATOGRAPHY LIB, V12, P159

[6]

YUAN ZY, 1981, ACTA BIOCH BIOPH SIN, V13, P291

← 1 →