MICROCHEMICAL STRUCTURAL DETERMINATION OF A PEPTOID COVALENTLY BOUND TO A POLYMERIC BEAD BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY

被引：53

作者：

ZAMBIAS, RA ^{[1
]}

BOULTON, DA ^{[1
]}

GRIFFIN, PR ^{[1
]}

机构：

[1] MERCK & CO INC,MERCK SHARP & DOHME RES LABS,DEPT ANALYT BIOCHEM,RAHWAY,NJ 07065

来源：

TETRAHEDRON LETTERS | 1994年 / 35卷 / 25期

关键词：

D O I：

10.1016/S0040-4039(00)73334-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

New methodology utilizing matrix-assisted laser desorption ionization time-of-flight mass spectroscopy (MALDI-TOF MS) for the direct identification of a ligand covalently attached to a polymeric bead is reported. The method allows for structure determination without relying on encoding strategies. Gaseous TFA cleavage of a peptoid from a single bead followed by mounting in MALDI matrix and irradiation with a beam at 337 nm gave abundant molecular ions and structurally informative fragment ions produced by post source decay (PSD).

引用

页码：4283 / 4286

页数：4

共 9 条

[1]

HILLENKAMP F, 1991, ANAL CHEM, V63, pA1193

[2] MULTIPLE PEPTIDE-SYNTHESIS METHODS AND THEIR APPLICATIONS [J].

JUNG, G ;

BECKSICKINGER, AG .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1992, 31 (04) :367-383

[3] MASS-SPECTROMETRIC SEQUENCING OF LINEAR PEPTIDES BY PRODUCT-ION ANALYSIS IN A REFLECTRON TIME-OF-FLIGHT MASS-SPECTROMETER USING MATRIX-ASSISTED LASER-DESORPTION IONIZATION [J].

KAUFMANN, R ;

SPENGLER, B ;

LUTZENKIRCHEN, F .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (10) :902-910

[4] SYNTHETIC METHODS FOR THE IMPLEMENTATION OF ENCODED COMBINATORIAL CHEMISTRY [J].

NIELSEN, J ;

BRENNER, S ;

JANDA, KD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (21) :9812-9813

[5]

NIKOLAIEV V, 1993, PEPTIDE RES, V6, P161

[6] COMPLEX SYNTHETIC CHEMICAL LIBRARIES INDEXED WITH MOLECULAR TAGS [J].

OHLMEYER, MHJ ;

SWANSON, RN ;

DILLARD, LW ;

READER, JC ;

ASOULINE, G ;

KOBAYASHI, R ;

WIGLER, M ;

STILL, WC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (23) :10922-10926

[7] THE GENERATION OF MOLECULAR DIVERSITY [J].

PAVIA, MR ;

SAWYER, TK ;

MOOS, WH .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1993, 3 (03) :387-396

[8] SOLID-PHASE SYNTHESIS OF PROTECTED PEPTIDE-FRAGMENTS USING A TRIALKOXY-DIPHENYL-METHYLESTER RESIN [J].

RINK, H .

TETRAHEDRON LETTERS, 1987, 28 (33) :3787-3790

[9]

ZUUCKERMANN R, 1992, J AM CHEM SOC, V114, P10646

← 1 →