ISOLATION OF GLUTATHIONE-DEFICIENT MUTANTS OF THE YEAST SACCHAROMYCES-CEREVISIAE

被引：51

作者：

KISTLER, M ^{[1
]}

SUMMER, KH ^{[1
]}

ECKARDT, F ^{[1
]}

机构：

[1] GESELL STRAHLEN & UMWELTFORSCH MBH, TOXIKOL ABT, D-8042 NEUHERBERG, GERMANY

来源：

MUTATION RESEARCH | 1986年 / 173卷 / 02期

关键词：

D O I：

10.1016/0165-7992(86)90087-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Glutathione-deficient (gsh-) mutants of the yeast Saccharomyces cerevisiae were isolated after UV treatment using MNNG as selective agent. For genetic and biochemical characterization 5 mutant strains were chosen which exhibited considerably decreased residual GSH contents varying from 2 to 6% of the wild-type levels. All 5 isolates showed a 2:2 segregation of the gsh-:GSH+ phenotypes alluding to a monogenic recessive mutation. Complementation analysis indicates that all gsh- mutants belong to one complementation group.

引用

页码：117 / 120

页数：4

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