EVALUATION OF A RAPID MEMBRANE-BASED ASSAY (HIV-CHEK) FOR DETECTION OF ANTIBODIES TO HIV IN SERUM SAMPLES FROM NAIROBI

被引：6

作者：

MOSS, GB

MAITHA, GM

WAMOLA, IA

NDINYAACHOLA, JO

PLUMMER, FA

KREISS, JK

机构：

[1] UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195

[2] UNIV WASHINGTON,DEPT EPIDEMIOL,SEATTLE,WA 98195

[3] UNIV NAIROBI,DEPT MED MICROBIOL,NAIROBI,KENYA

[4] UNIV MANITOBA,DEPT MED MICROBIOL,WINNIPEG R3T 2N2,MANITOBA,CANADA

来源：

AIDS | 1990年 / 4卷 / 04期

关键词：

AIDS serodiagnosis; HIV; HIV antibodies;

D O I：

10.1097/00002030-199004000-00011

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We evaluated a rapid membrane-based assay (HIV-CHEK) for detection of antibodies to HIV using 737 serum samples in Nairobi, Kenya. The rapid assay had a sensitivity of 96.3% and specificity of 99.8% when compared with enzyme-linked immunosorbent assay (ELISA) and Western blot assay. Results were similar using fresh or previously frozen serum samples, although the latter occasionally left debris on the assay device membrane yielding uninterpretable results. This rapid HIV assay may be of particular use in developing countries where laboratory resources are limited.

引用

页码：351 / 353

页数：3

共 11 条

[1]

CARLSON JR, 1987, LANCET, V1, P361

[2] DIAGNOSTIC USEFULNESS OF 5 SCREENING ASSAYS FOR HIV IN AN EAST-AFRICAN CITY WHERE PREVALENCE OF INFECTION IS LOW [J].

CONSTANTINE, NT ;

FOX, E ;

ABBATTE, EA ;

WOODY, JN .

AIDS, 1989, 3 (05) :313-317

[3] THE ASSOCIATION BETWEEN MALARIA, BLOOD-TRANSFUSIONS, AND HIV SEROPOSITIVITY IN A PEDIATRIC POPULATION IN KINSHASA, ZAIRE [J].

GREENBERG, AE ;

NGUYENDINH, P ;

MANN, JM ;

KABOTE, N ;

COLEBUNDERS, RL ;

FRANCIS, H ;

QUINN, TC ;

BAUDOUX, P ;

LYAMBA, B ;

DAVACHI, F ;

ROBERTS, JM ;

KABEYA, N ;

CURRAN, JW ;

CAMPBELL, CC .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1988, 259 (04) :545-549

[4] AIDS - AN INTERNATIONAL PERSPECTIVE [J].

PIOT, P ;

PLUMMER, FA ;

MHALU, FS ;

LAMBORAY, JL ;

CHIN, J ;

MANN, JM .

SCIENCE, 1988, 239 (4840) :573-579

[5] RAPID LATEX AGGLUTINATION ASSAY USING RECOMBINANT ENVELOPE POLYPEPTIDE FOR THE DETECTION OF ANTIBODY TO THE HIV [J].

QUINN, TC ;

RIGGIN, CH ;

KLINE, RL ;

FRANCIS, H ;

MULANGA, K ;

SENSION, MG ;

FAUCI, AS .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1988, 260 (04) :510-513

[6]

SPIELBERG F, 1989, LANCET, V1, P580

[7] COMPARISON OF 6 SEROLOGICAL ASSAYS FOR HUMAN IMMUNODEFICIENCY VIRUS-ANTIBODY DETECTION IN DEVELOPING-COUNTRIES [J].

VANDEPERRE, P ;

NZARAMBA, D ;

ALLEN, S ;

RIGGIN, CH ;

SPRECHERGOLDBERGER, S ;

BUTZLER, JP .

JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (03) :552-556

[8]

VANDEPERRE P, 1988, 3 INT C AIDS STOCKH

[9]

1989, LANCET, V1, P941

[10]

1988, MMWR, V36, P833

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