BINDING OF 4'-AMINOMETHYL 4,5',8-TRIMETHYL PSORALEN TO DNA, RNA AND PROTEIN IN HELA-CELLS AND DROSOPHILA CELLS

In Drosophila cells and HeLa cells treated with 4′-aminomethyl trioxsalen and ultraviolet light, this compound binds covalently to DNA and RNA. The maximum number of molecules bound to 103 base pairs in DNA is 60 and in RNA it is 20. In nuclei treated likewise the number of molecules bound to 103 base pairs in DNA can be as high as 376. When cells are irradiated in the frozen state the number of 4′-aminomethyl trioxsalen molecules bound per 103 base pairs in DNA is about 40 and in RNA about 20. DNA molecules from cells or nuclei treated with 4′-aminomethyl trioxsalen and ultraviolet light are highly crosslinked and appear as loops interspersed by double stranded regions when analyzed in the electron microscope under denaturing conditions. The loop sizes are heterogeneous and the fraction of double stranded regions increases to almost complete double-strandedness at high degrees of reaction. No sècondary structures could be found in ribosomal RNA from Drosophila cells or HeLa cells after treatment with 4′-aminomethyl trioxsalen and ultraviolet light. In cells treated with 4′-aminomethyl trioxsalen and ultraviolet light the RNAase activity is increased considerably suggesting a release of lysosomal enzymes. 4′-aminomethyl trioxsalen and its photodecomposition products bind strongly to cellular proteins. © 1979.