WATER TRANSPORT IN HUMAN RED-CELLS - EFFECTS OF NONINHIBITORY SULFHYDRYL-REAGENTS

被引：17

作者：

BENGA, G

POPESCU, O

BORZA, V

HODARNAU, A

POP, VI

WRIGGLESWORTH, J

机构：

[1] INST HYG & PUBL HLTH, Cluj Napoca, ROMANIA

[2] UNIV LONDON KINGS COLL, DEPT BIOCHEM, LONDON WC2R 2LS, ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1061卷 / 02期

基金：

英国惠康基金;

关键词：

WATER DIFFUSION; RED BLOOD CELL; SULFHYDRYL REAGENT; NMR; (HUMAN);

D O I：

10.1016/0005-2736(91)90297-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The water diffusional permeability of human red blood cells following exposure to various sulfhydryl group (SH) reagents have been studied using a nuclear magnetic resonance technique. Exposure of red blood cells up to 12 mM N-ethylmaleimide (NEM) or 10 mM 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNE) alone does not affect water diffusion. In contrast, when DTNB treatment follows a preincubation of the cells with NEM, a small (18% at 37-degrees-C) but significant inhibition of water permeability occurs. The NEM and DTNB treatment of the cells caused no change of the cell shape and volume or of the cell water volume. Consequently, the inhibition observed after NEM and DTNB treatment has a real significance.

引用

页码：309 / 312

页数：4

共 26 条

[1] STUDIES OF WATER PERMEABILITY AND PROTEINS OF ERYTHROCYTE-MEMBRANES IN PATIENTS WITH DUCHENNE MUSCULAR-DYSTROPHY [J].