EQUILIBRIA OF BIOMOLECULES ON ION-EXCHANGE ADSORBENTS

被引：27

作者：

JAMES, EA ^{[1
]}

DO, DD ^{[1
]}

机构：

[1] UNIV QUEENSLAND,DEPT CHEM ENGN,ST LUCIA,QLD 4072,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 542卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)88745-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Equilibrium adsorption isotherms of bovine serum albumin on DEAE-Sepharose Fast Flow ion exchanger were studied with different salt concentrations and buffer strengths at varying pH using a batch method. An extended Langmuir-Freundlich isotherm equation was used to fit these data and other experimental data taken from the literature. It was found that the model correctly approximates the adsorption of various proteins and amino acids at different salt concentrations using a single set of parameters.

引用

页码：19 / 28

页数：10

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