INHERITANCE OF A CO-TRANSFERRED FOREIGN GENE IN THE PROGENIES OF TRANSGENIC RICE PLANTS

被引:38
作者
GOTO, F
TOKI, S
UCHIMIYA, H
机构
[1] UNIV TOKYO, INST MOLEC & CELLULAR BIOSCI, BUNKYO KU, TOKYO 113, JAPAN
[2] HOKKAIDO UNIV, DEPT BOT, SAPPORO, HOKKAIDO 060, JAPAN
关键词
HYGROMYCIN PHOSPHOTRANSFERASE; BETA-GLUCURONIDASE; RICE (ORYZA-SATIVA L); ELECTROPORATION; INHERITANCE;
D O I
10.1007/BF01968842
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The integration pattern and the inheritance of exogenous DNA in transgenic rice plants were analysed. Plasmid pCH (4.8 kb), that contains chimaeric cauliflower mosaic virus 35S promoter-hygromycin phosphotransferase structural gene, and plasmid pGP400 (7.2 kb), possessing oat phytochrome promoter and structural gene of bacterial beta-glucuronidase, were co-transferred into protoplasts of rice (Oryza sativa L.) plants via electroporation. Primary transformants (T0 generation) and their progenies (T1, T2 and T3) were selected by hygromycin B. Southern blot analysis of inserted genes in transgenic rice plants suggests the integration of an intact hygromycin phosphotransferase gene and non-functional DNA fragments into host genome. Co-inheritance of the hygromycin phosphotransferase gene and beta-glucuronidase gene was also observed. There were no significant differences in terms of the morphology and size of seeds between untransformed and transgenic plants (T3 generation).
引用
收藏
页码:300 / 305
页数:6
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