WORTMANNIN, A SPECIFIC PHOSPHATIDYLINOSITOL 3-KINASE INHIBITOR, INHIBITS ADIPOCYTIC DIFFERENTIATION OF 3T3-L1 CELLS

被引：103

作者：

TOMIYAMA, K

NAKATA, H

SASA, H

ARIMURA, S

NISHIO, E

WATANABE, Y

机构：

[1] Department of Pharmacology, National Defense Medical College, Tokorazawa 359

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 212卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1965

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of wortmannin, which inhibits phosphatidylinositol 3-kinase (PI 3-kinase), on adipocytic differentiation of 3T3-L1 cells was examined. The extent of differentiation was evaluated by the staining of adipocytes with Oil Red O and measurement of glycerol 3-phosphate dehydrogenase (GPDH) activity. Wortmannin, at over 100 nM, significantly inhibited adipogenesis of cells treated with isobutyl-methylxanthine, dexamethasone, and insulin with an IC50 value about 50 nM. These results suggest that PI 3-kinase plays a role in adipocytic differentiation of 3T3-L1 cells. (C) 1995 Academic Press, Inc.

引用

页码：263 / 269

页数：7

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