IMMUNOLOGICAL ASSAYS ON THE RELATION OF CULTURE-MEDIUM PEROXIDASES TO THOSE IN FRACTIONS-ISOLATED FROM PEANUT CELLS

Through the means of Ouchterlony gel diffusion, Western blot and competitive ELISA it was determined that A. PRX or C. PRX from culture medium and their corresponding isozymes from the cytoplasm and cell wall have a higher than 80 % similarity. Quantitative assays indicated that the cell-wall fractions were largely represented by the major cationic peroxidase isolated from spent medium of peanut cells. Conversely the cytoplasmic- or soluble-cell fraction was mainly represented by the anionic isozyme isolated from that medium. Consequently the enriched medium peroxidases may be used for studies of events in the cytoplasm and cell wall.