QUALITY OF OOCYTES FROM SUPEROVULATED RHESUS-MONKEYS

被引：15

作者：

JOHNSON, LD

MATTSON, BA

ALBERTINI, DF

SEHGAL, PK

BECKER, RA

AVIS, J

BIGGERS, JD

机构：

[1] New England Regional Primate Research Center (NERPRC), Harvard Medical School, Southborough

[2] Laboratory for Human Reproduction and Reproductive Biology (LHRRB), Department of Physiology, Harvard Medical School, Boston

[3] Department of Anatomy and Cellular Biology, Tufts University Health Science Center, Boston

[4] La Jolla Cancer Institute, La Jolla, CA

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 05期

关键词：

OOCYTE MATURATION; RHESUS; SUPEROVULATION;

D O I：

10.1093/oxfordjournals.humrep.a137395

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The parameters, serum oestradiol (E2) response, follicle size and cumulus morphology, which are commonly used to determine in-vivo oocyte maturation in human in-vitro fertilization and embryo transfer (IVF-ET) programmes, were shown to be unreliable predictors of maturation of rhesus oocytes. In two groups of rhesus, one stimulated with pregnant mares' serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) and the other with human menopausal gonadotrophin (HMG) and HCG, the three parameters varied widely within and between protocols. Triple fluorochrome staining (TFS) for chromatin, microtubules and filamentous actin (f-actin) in oocytes at the time of collection and following 24 h in culture showed major differences in their maturation both in vivo and in vitro following priming with PMSG and HMG. In evaluating IVF protocols, TFS provides a valuable assay for the meiotic status of fixed oocytes of non-human primates.

引用

页码：623 / 631

页数：9

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