MOLECULAR-CLONING OF RAT MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) AND ITS EXPRESSION IN RAT SPLEEN-CELLS AND TUMOR-CELL LINES

被引：140

作者：

YOSHIMURA, T ^{[1
]}

TAKEYA, M ^{[1
]}

TAKAHASHI, K ^{[1
]}

机构：

[1] KUMAMOTO UNIV,SCH MED,DEPT PATHOL,KUMAMOTO 860,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91445-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat monocyte chemoattractant protein-1 (MCP-1) cDNA was cloned. A cDNA library was constructed from mRNA extracted from Con A-stimulated rat spleen cells. After 2 rounds of screening with a radiolabeled oligonucleotide probe and DNA sequencing from plasmid DNAs, one clone which encoded for MCP-1 was obtained. The cDNA clone comprises 665 base pairs with an open reading frame which encodes for 148 amino acid protein. Spleen cells expressed a significant amount of MCP-1 mRNA without stimulus. But higher expression was observed when cells were stimulated with Con A. MCP-1 mRNA was also expressed in tumor cell lines, although the amount of expressed MCP-1 mRNA was different among cell lines. © 1991.

引用

页码：504 / 509

页数：6

共 20 条

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