FOOT-SHOCK STRESS ENHANCES THE INCREASE OF [S-35] TBPS BINDING IN THE RAT CEREBRAL-CORTEX AND THE CONVULSIONS INDUCED BY ISONIAZID

We report earlier that isoniazid and foot-shock stress individually increase the maximal number of [S-35]TBPS binding sites (B(max)) measured "ex vivo" in unwashed membranes from rat cerebral cortex and that the increase due to both treatments are prevented by pretreatment "in vivo" with diazepam which alone induced a significant decrease in the total number of [S-35]TBPS binding sites. In the present paper, the effect of stress was studied on both the increase in [S-35]TBPS binding and the convulsant activity induced by isoniazid in unstressed rats. Isoniazid induced a time dependent increase in [S-35]TBPS binding. The isoniazid-induced increase in [S-35]TBPS binding was markedly potentiated by foot-shock stress. Moreover, foot-shock stress markedly reduced the latency to the appearance of generalized seizures induced by isoniazid (300 mg/kg s.c.). The results provide evidence that the "in vivo" inhibition of GABAergic transmission elicited by isoniazid results in an increase of [S-35]TBPS binding in the rats cerebral cortex. The finding that stress, like isoniazid, enhances [S-35]TBPS binding suggests that this treatment also inhibits the function of GABAergic synapses.