PROMOTER FOR THE HUMAN FERRITIN HEAVY CHAIN-ENCODING GENE (FERH) - STRUCTURAL AND FUNCTIONAL-CHARACTERIZATION

被引：34

作者：

BEVILACQUA, MA ^{[1
]}

GIORDANO, M ^{[1
]}

DAGOSTINO, P ^{[1
]}

SANTORO, C ^{[1
]}

CIMINO, F ^{[1
]}

COSTANZO, F ^{[1
]}

机构：

[1] UNIV NAPOLI FEDERICO 2,DIPARTIMENTO BIOCHIM & BIOTECNOL MED,VIA S PANSIN 5,I-80131 NAPLES,ITALY

来源：

GENE | 1992年 / 111卷 / 02期

关键词：

RECOMBINANT DNA; GENE REGULATION; TRANSCRIPTION FACTOR SP1; DNA FOOTPRINTING; CHLORAMPHENICOL ACETYLTRANSFERASE ASSAY;

D O I：

10.1016/0378-1119(92)90696-M

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We conducted a functional analysis of the promoter for the human ferritin heavy chain-encoding gene (pFERH) in HepG2 and HeLa cells. The activity of pFERH is equivalent in both cell types, despite their different ferritin (Fer) isotypes. Transfections of a series of 5'-deletion mutants indicate that pFERH activity is essentially dependent on two motifs. One of them, accounting for about 50% of the total transcriptional activity, is recognized by the RNA polymerase II transciption factor, Sp1, and the other by a low-affinity factor present in both the cell types analyzed.

引用

页码：255 / 260

页数：6

共 26 条

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