ROLE OF CALCIUM AND DIFFERENTIATION IN ENHANCED SESQUITERPENE ELICITATION FROM CALCIUM ALGINATE-IMMOBILIZED PLANT-TISSUE

被引：16

作者：

CURTIS, WR ^{[1
]}

WANG, P ^{[1
]}

HUMPHREY, A ^{[1
]}

机构：

[1] PENN STATE UNIV,INST BIOTECHNOL,UNIVERSITY PK,PA 16802

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 06期

关键词：

IMMOBILIZATION; ROOT CULTURE; FUNGAL ELICITATION; HYDRODYNAMIC STRESS; SECONDARY METABOLITES; SIGNAL TRANSDUCTION;

D O I：

10.1016/0141-0229(94)00061-U

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Calcium alginate immobilization of Hyoscyamus muticus plant tissue culture is shown to enhance the rapid formation of sesquiterpenes in response to exposure to extracts of the fungal pathogen Rhizoctonia solani. The studies were carried out with suspension-cultured cells and roots at low tissue loadings to demonstrate that the enhancement is not due to either the degree of differentiation or the presence of metabolic gradients. The fungally elicited response of root tissue is shown to be much less sensitive to both the physical and chemical environment than free cell suspensions. By examining the effects of the immobilization components (Ca2+, alginate), and immobilization in barium alginate gels, it is concluded that the enhanced sesquiterpene production in immobilized tissue is mediated through calcium.

引用

页码：554 / 557

页数：4

共 26 条

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