STIMULATORY EFFECTS OF AMINO-ACIDS AND OLEIC-ACID ON POLY(3-HYDROXYBUTYRIC ACID) SYNTHESIS BY RECOMBINANT ESCHERICHIA-COLI

被引：44

作者：

LEE, SY

LEE, YK

CHANG, HN

机构：

[1] Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, Taejon, 305-701, 373-1 Kusong-dong, Yusong-gu

[2] BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Taejon, 305-701, 373-1 Kusong-dong, Yusong-gu

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1995年 / 79卷 / 02期

关键词：

POLY(3-HYDROXYBUTYRIC ACID); PHB; ESCHERICHIA COLI; AMINO ACID; OLEIC ACID;

D O I：

10.1016/0922-338X(95)94089-A

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Addition of cysteine, isoleucine, methionine, or proline promoted poly(3-hydroxybutyric acid) [PHB] synthesis by recombinant Escherichia coli more than two-fold. Oleic acid also enhanced PHB synthesis more than three-fold. A PHB concentration of 70 g/l could be obtained by fed-batch culture of recombinant E. coli in a defined medium supplemented with small amounts of isoleucine, methionine, and proline. The stimulatory effects of amino acids and oleic acid on PHB synthesis seems to be due to the availability of more acetyl-CoA and/or NADPH.

引用

页码：177 / 180

页数：4

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