OPTICAL BIOSENSOR FOR MONITORING MICROBIAL-CELLS

被引:118
作者
WATTS, HJ
LOWE, CR
POLLARDKNIGHT, DV
机构
[1] UNIV CAMBRIDGE,INST BIOTECHNOL,CAMBRIDGE CB1 2QT,CAMBS,ENGLAND
[2] FISONS APPL SENSOR TECHNOL,CAMBRIDGE CB3 8SL,CAMBS,ENGLAND
关键词
D O I
10.1021/ac00087a010
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The potential of a new optical biosensor, the resonant mirror, for detecting whole cells is demonstrated. Staphylococcus aureus (Cowan-1) cells, which express protein-A at their surface, were detected by binding to human immunoglobulin G (IgC) immobilized on an aminosilane-derivatized sensor surface at concentrations in the range 8 x 10(6)-8 x 10(7) cells/mL. A control S. aureus strain (Wood-46), which does not express protein-A, gave no significant response. Immobilization of the capture ligand on aminosilane surfaces with and without a hydrogel coating of carboxymethyl-dextran was compared. The greatest binding response was observed with non-dextran-coated surfaces. The sensitivity of the technique was increased a 1000-fold by using a human IgG-colloidal gold conjugate (30 nm) in a sandwich assay format. S. aureus (Cowan-1) cells were detected in spiked milk samples at cell concentrations from 4 x 10(3)-1.6 x 10(6) cells/mL using the sandwich assay.
引用
收藏
页码:2465 / 2470
页数:6
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