POLYMERASE CHAIN-REACTION OF WHOLE-CELL LYSATES FOR THE DETECTION OF VIBRIO-VULNIFICUS

被引：8

作者：

BRAUNS, LA

OLIVER, JD

机构：

[1] Department of Biology, University of North Carolina at Charlotte, Charlotte, NC

来源：

FOOD BIOTECHNOLOGY | 1994年 / 8卷 / 01期

关键词：

D O I：

10.1080/08905439409549865

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A quantitative, efficient, and rapid method of detecting cells of the human pathogen, Vibrio vulnificus, using the polymerase chain reaction is described. The technique employs whole cell lysates as the source of template DNA, and amplifies the cytotoxin/hemolysin gene. Using primers internal to this gene, we were able to detect a single cell of V. vulnificus. This method, which does not require DNA extraction or use of a probe, may facilitate the detection of low levels of this pathogen when present in shellfish.

引用

页码：1 / 6

页数：6

共 7 条

[1] USE OF THE POLYMERASE CHAIN-REACTION IN DETECTION OF CULTURABLE AND NONCULTURABLE VIBRIO-VULNIFICUS CELLS
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[3] IDENTIFICATION OF ENVIRONMENTAL VIBRIO-VULNIFICUS ISOLATES WITH A DNA PROBE FOR THE CYTOTOXIN-HEMOLYSIN GENE
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OLIVER, JD
GUTHRIE, K
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[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (02) : 737 - 739
[6] THE CYTOLYSIN GENE OF VIBRIO-VULNIFICUS - SEQUENCE AND RELATIONSHIP TO THE VIBRIO-CHOLERAE EL-TOR HEMOLYSIN GENE
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[7] 1989, ESTIMATES RELATIVE R

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