SIMPLE APPARATUS FOR PERFORMING SHORT-TIME (1-2 SECONDS) UPTAKE MEASUREMENTS IN SMALL VOLUMES - ITS APPLICATION TO D-GLUCOSE TRANSPORT STUDIES IN BRUSH-BORDER VESICLES FROM RABBIT JEJUNUM AND ILEUM

An automated procedure allows uptake measurements with incubation times as short as 0.5 s and with volumes of 10-20 μl. Using this technique the kinetic parameters Km and V of d-glucose transport in brush border vesicles from rabbit small intestine could be determined from unidirectional fluxes. A comparison of the data obtained from jejunum and from ileum shows that the Km for d-glucose is the same in both parts of the intestine, whereas the maximum flux is significantly larger in the jejunum. © 1978.