URIDINE-DIPHOSPHATE GALACTOSE-4 EPIMERASE OF FENUGREEK - PURIFICATION TESTS AND SOME PROPERTIES

被引:4
作者
CLERMONT, S
PERCHERON, F
机构
[1] Laboratoire de Chimie Biologique, Faculté des Sciences Pharmaceutiques et Biologiques, Université René Descartes, Paris
关键词
D O I
10.1016/S0031-9422(00)82712-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The presence of a UDP Gal-4 epimerase is reported in fenugreek seedlings. It occurs as a soluble enzyme which loses its activity at pHs lower than 7, with an optimum pH at 8.5. Partial purification was achieved by ammonium sulphate precipitation and hydroxyapatite chromatography. The MW is 70 000 and it is linked to NAD+ as in the case of the enzymes of E. coli and yeast. © 1979.
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页码:1963 / 1965
页数:3
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