TRANSPORT OF L-CYSTEINESULFINATE IN RAT-LIVER MITOCHONDRIA

1. 1. The mechanism of l-cysteinesulfinate permeation into rat liver mitochondria has been investigated. 2. 2. Mitochondria do not swell in ammonium or potassium salts of l-cysteinesulfinate in all the conditions tested, including the presence of valinomycin and/or carbonylcyanide p-trifluoromethoxyphenylhydrazone. 3. 3. The activation of malate oxidation by l-cysteinesulfinate is abolished by aminooxyacetate, an inhibitor of the intramitochondrial aspartate aminotransferase, it is not inhibited by high concentrations of carbonylcyanide p-trifluoromethoxyphenylhydrazone (in contrast to the oxidation of malate plus glutamate) and it is decreased on lowering the pH of the medium. 4. 4. All the aspartate formed during the oxidation of malate plus l-cysteine-sulfinate is exported into the extramitochondrial space. 5. 5. Homocysteinesulfinate, cysteate and homocysteate, which are all good substrates of the mitochondrial aspartate aminotransferase, are unable to activate the oxidation of malate. Homocysteinesulfinate and homocysteate have no inhibitory effect on the l-cysteinesulfinate-induced respiration, whereas cysteate inhibits it competitively with respect to l-cysteinesulfinate. 6. 6. In contrast to d-aspartate, d-cysteinesulfinate and d-glutamate, l-aspartate inhibits the oxidation of malate plus l-cysteinesulfinate in a competitive way with respect to l-cysteinesulfinate. Vice versa, l-cysteinesulfinate inhibits the influx of l-aspartate. 7. 7. Externally added l-cysteinesulfinate elicits efflux of intramitochondrial l-aspartate or l-glutamate. The cysteinesulfinate analogues homocysteinesulfinate, cysteate and homocysteate and the d-stereoisomers of cysteinesulfinate, aspartate and glutamate do not cause a significant release of internal glutamate or aspartate, indicating a high degree of specifity of the exchange reactions. External l-cysteinesulfinate does not cause efflux of intramitochondrial Pi, malate, malonate, citrate, oxoglutarate, pyruvate or ADP. The l-cysteinesulfinate-aspartate and l-cysteinsulfinate-glutamate exchanges are inhibited by glisoxepide and by known substrates of the glutamate-aspartate carrier. 8. 8. The exchange between external l-cysteinesulfinate and intramitochondrial glutamate is accompanied by translocation of protons across the mitochondrial membrane in the same direction as glutamate. The l-cysteinesulfinate-aspartate exchange, on the other hand, is not accompanied by H+ translocation. 9. 9. The ratios Δl-cysteinesulfinate/Δaspartate are close to unity, 10. 10. It is concluded that l-cysteinesulfinate is transported by the glutamate-aspartate carrier of rat liver mitochondria. The present data suggest that the dissociated form of l-cysteinesulfinate exchanges with H+-compensated glutamate or with negatively charged aspartate. © 1979.