COMPONENT OF THE ALTERNATIVE OXIDASE LOCALIZED TO THE MATRIX SURFACE OF THE INNER MEMBRANE OF PLANT-MITOCHONDRIA

被引:34
作者
RASMUSSON, AG
MOLLER, IM
PALMER, JM
机构
[1] UNIV LUND,DEPT PLANT PHYSIOL,BOX 7007,S-22007 LUND 7,SWEDEN
[2] IMPERIAL COLL SCI TECHNOL & MED,DEPT PURE & APPL BIOL,LONDON SW7 2BB,ENGLAND
关键词
(Arum maculatum; Plant; Mitochondria); Alternative pathway; Inside-out submitochondrial particle; Mitoplast; Trypsin;
D O I
10.1016/0014-5793(90)80034-G
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In mitoplasts from Arum maculatum spadices, succinate dehydrogenase (EC 1.3.99.1) and the alternative, cyanide-resistant oxidase activity (measured as m-chlorobenzhydroxamic acid-sensitive duroquinol oxidation) was unaffected by treatment with trypsin. In contrast, when 85% inside-out submitochondrial particles were treated with trypsin the alternative oxidase activity was inhibited by about 50% and succinate dehydrogenase activity by about 40%. Thus, a trypsin-sensitive component of the alternative pathway is located on the inner surface of the inner mitochondrial membrane. After trypsin treatment of the inside-out submitochondrial particles the inhibited alternative oxidase activity was partly restored by including 0.7 M citrate in the assay medium. This indicates that trypsin does not destroy the active site but merely causes a conformational change in the enzyme, thereby lowering its activity. © 1990.
引用
收藏
页码:311 / 314
页数:4
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