SPATIAL-DISTRIBUTION OF FLAVONOID CONJUGATES IN RELATION TO GLUCOSYLTRANSFERASE AND SULFOTRANSFERASE ACTIVITIES IN FLAVERIA-BIDENTIS

被引:13
作者
HANNOUFA, A [1 ]
VARIN, L [1 ]
IBRAHIM, RK [1 ]
机构
[1] CONCORDIA UNIV,DEPT BIOL,PLANT BIOCHEM LAB,MONTREAL H3G 1M8,QUEBEC,CANADA
关键词
D O I
10.1104/pp.97.1.259
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The spatial distribution of sulfated and glucosylated flavonols as well as of the enzymes involved in the later steps of their biosynthesis, sulfotransferase and glucosyltransferase, were investigated in the shoots of Flaveria bidentis. The highest amounts of both types of flavonoid conjugates (as micromole per gram fresh weight) and the highest activities of their enzymes (as picokat per milligram) were detected in the terminal bud and the first pair of leaves. Sulfotransferase activity was also highest in the upper stem segments and in the basal section of the leaves. Western blot analysis of protein extracts showed that variations in sulfotransferase activity in different tissues correlate well with the amounts of immunodetected enzyme protein. These results were discussed in relation to the possible role of conjugated flavonoids in plant growth.
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页码:259 / 263
页数:5
相关论文
共 15 条
  • [1] Anderson J. W., 1980, The biochemistry of plants. A comprehensive treatise. Volume 5. Amino acids and derivatives., P203
  • [2] PURIFICATION AND PROPERTIES OF FLAVONOL-RING-B GLUCOSYLTRANSFERASE FROM CHRYSOSPLENIUM-AMERICANUM
    BAJAJ, KL
    DELUCA, V
    KHOURI, H
    IBRAHIM, RK
    [J]. PLANT PHYSIOLOGY, 1983, 72 (03) : 891 - 896
  • [3] SULFATED FLAVONOIDS - AN UPDATE
    BARRON, D
    VARIN, L
    IBRAHIM, RK
    HARBORNE, JB
    WILLIAMS, CA
    [J]. PHYTOCHEMISTRY, 1988, 27 (08) : 2375 - 2395
  • [4] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [5] ESAU K, 1965, PLANT ANATOMY, V2
  • [6] FLAVONOID SULFATES - NEW CLASS OF SULFUR-COMPOUNDS IN HIGHER-PLANTS
    HARBORNE, JB
    [J]. PHYTOCHEMISTRY, 1975, 14 (5-6) : 1147 - 1155
  • [7] HARBORNE JB, 1988, B LIAISON GROUPE POL, V14, P3
  • [8] HELLER W, 1988, ADV RES 1980, P399
  • [9] NATURALLY-OCCURRING AUXIN TRANSPORT REGULATORS
    JACOBS, M
    RUBERY, PH
    [J]. SCIENCE, 1988, 241 (4863) : 346 - 349
  • [10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
    LAEMMLI, UK
    [J]. NATURE, 1970, 227 (5259) : 680 - +