THE CALMODULIN ANTAGONIST W-7 DEPLETES INTRACELLULAR CALCIUM STORES IN FRTL-5 THYROID-CELLS

被引：15

作者：

TORNQUIST, K ^{[1
]}

机构：

[1] UNIV HELSINKI,ENDOCRINE RES LAB,SF-00100 HELSINKI 10,FINLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 190卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.1007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubating Fura 2 loaded thyroid FRTL-5 cells with the calmodulin inhibitor W-7 decreased the ATP-evoked increase in intracellular free calcium. In addition, pretreatment of the cells with W-7 decreased both the thapsigargin-evoked release of sequestered calcium and the entry of extracellular calcium. Studies with 45Ca2+ showed that W-7 decreased the amount of sequestered calcium in the cells. Furthermore, after stimulating with ATP, the ability to sequester calcium was decreased in cells treated with W-7 compared with control cells. The results suggest that calmodulin is necessary for the signal-transduction system in FRTL-5' cells, and may be especially important in maintaining functional intracellular calcium stores in the cells. © 1993 Academic Press, Inc.

引用

页码：37 / 41

页数：5

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