THE REGULATION OF THE INTRACELLULAR SODIUM-ION CONCENTRATION IN CULTURED CHICK-EMBRYO HEART-CELLS

被引：1

作者：

AHLEMEYER, B ^{[1
]}

WEINTRAUT, H ^{[1
]}

SCHONER, W ^{[1
]}

机构：

[1] UNIV GIESSEN,ZENTRUM STRAHLEN,W-6300 GIESSEN,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 186卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90799-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using digital imaging microscopy with the fluorescent indicator sodium-binding benzofuran isophtalate, we examined the cytosolic Na+ concentration ([Na+]i) in individual chick embryo heart cells. Inhibition of the Na+-H+ exchanger using Na+-free (Li+ substituted) medium and inhibition of the Na+-efflux through the Na+-Ca2+ exchanger using Ca2+-free medium didn't change the [Na+]i. The opening of voltage-dependent Na+ channels with veratridine (150 μg/ml) and inhibition of the Na+-K+-Cl--cotransporter with bumetanide (10 μM) led to an increase in [Na+]i by 107% and 86%, respectively, suggesting that the Na+ channels and the Na+-K+-Cl- cotransporter predominantly regulate the [Na+]i in cultured chick embryo heart cells. © 1992.

引用

页码：674 / 679

页数：6

共 23 条

[1] CHICK HEART-CELLS WITH HIGH INTRACELLULAR CALCIUM-CONCENTRATION HAVE A HIGHER AFFINITY FOR CARDIAC-GLYCOSIDES THAN THOSE WITH LOW INTRACELLULAR CALCIUM-CONCENTRATION, AS REVEALED BY AFFINITY LABELING WITH A DIGOXIGENIN DERIVATIVE [J].