DIRECT-DETECTION OF BETA-1,3-GLUCANASE IN PLANT-EXTRACTS BY POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：8

作者：

KALIX, S ^{[1
]}

BUCHENAUER, H ^{[1
]}

机构：

[1] UNIV HOHENHEIM,INST PHYTOMED,D-70593 STUTTGART,GERMANY

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 06期

关键词：

NICOTIANA TABACUM; ACQUIRED RESISTANCE; GLUCANASE;

D O I：

10.1002/elps.11501601170

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

By using carboxymethyl (CM)-curdlan, a polysaccharide linked with the dye Remazol Brilliant Blue (RBB) as a substrate in polyacrylamide gels, the beta-1,3-glucanase in plant extracts can be detected directly by native polyacrylamide gel electrophoresis. In contrast to the usually used procedures for the detection of glucanases, e.g., colorimetric assay, overlay technique, enzyme activity staining using laminarin as a substrate, this method is rapid and allows both the determination of the activity and the location of the relative position of the multiple forms of beta-1,3-glucanases.

引用

页码：1016 / 1018

页数：3

共 16 条

[1] SOLUBLE CHROMOGENIC SUBSTRATES FOR THE ASSAY OF ENDO-1,4-BETA-XYLANASES AND ENDO-1,4-BETA-GLUCANASES [J].