CHOICE OF AN AQUEOUS POLYMER 2-PHASE SYSTEM FOR CELL PARTITION

被引：16

作者：

MIHEEVA, LM

ZASLAVSKY, BY

ROGOZHIN, SV

机构：

[1] Institute of Elementoorganic Compounds, Academy of Sciences, the U.S.S.R., Moscow

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 542卷 / 01期

关键词：

D O I：

10.1016/0304-4165(78)90236-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Partition of human erythrocytes in aqueous two-phase polymer systems produced by Ficoll and different molecular weight fractions of dextran and polyethylene glycol and the influence of the ionic composition on the cells' partition in the systems was studied. It is found that the Ficoll-dextran-40 system is characterized by a number of advantages as compared with the common dextran-polyethylene glycol system or the others systems under study. The main advantage of the system appears to be that it is possible to concentrate the red cells in the top phase or in the bottom phase of the system, depending on the system ionic composition. The influence of the nature and the concentration of salt additives on this two-phase system formation is examined. © 1978.

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页码：101 / 106

页数：6

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