AN ENDOGENOUS MODULATOR OF N-METHYL-D-ASPARTATE RECEPTOR-COUPLED GLYCINE RECEPTORS

Extensive washing of a membrane preparation from rat brain resulted in a progressive enhancement of strychnine-insensitive [H-3]glycine binding, which was due to an increase in the number of binding sites with no changes in the apparent affinity of this radioligand, precluding an explanation based solely on the elimination of endogenous glycine. Moreover, after extensive washing a population of [H-3]glycine binding sites with very high affinity for L-serine was observed in addition to the sites with low affinity for L-serine present in less extensively washed tissue. The observed changes in [H-3]glycine binding were attributable to the elimination of a low molecular weight, heat-stable compound which was readily detected in the wash supernatant. Extensive washing also altered [H-3](+)-5-methyl-10,11-dihydro-5H-dibenzo-[a,d]cyclohept-5,10-imine maleate ([H-3]MK-801) binding to N-methyl-D-aspartate (NMDA) receptor-associated channels, decreasing basal binding at equilibrium and producing slower association rates in the presence of either glycine or L-glutamate. Moreover, in well-washed membranes both glycine and glutamate enhanced [H-3]MK-801 binding acting at high- and low-affinity sites. These findings suggest that the NMDA receptor complex can assume interconverting conformational states regulated by an endogenous substance(s).