RAPID METHOD FOR THE ISOLATION OF KIDNEY BRUSH-BORDER MEMBRANES

被引：219

作者：

MALATHI, P

PREISER, H

FAIRCLOUGH, P

MALLETT, P

CRANE, RK

机构：

[1] College of Medicine and Dentistry of New Jersey, Rutgers Medical School, Department of Physiology and Biophysics, Piscataway

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 554卷 / 01期

基金：

美国国家科学基金会; 英国医学研究理事会; 美国国家卫生研究院;

关键词：

(Rabbit kidney); Alanine transport; Brush border membrane; Glucose transport; Membrane enzyme;

D O I：

10.1016/0005-2736(79)90023-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple rapid method for the preparation of purified brush border membranes from rabbit kidney proximal tubules is described. The method is based on hypotonic lysis, Ca2+ aggregation of contaminants and differential centrifugation. In contrast to most other published methods, the brush border membranes are free of contamination by basolateral membranes. © 1979.

引用

页码：259 / 263

页数：5

共 12 条

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