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GAS-LIQUID-CHROMATOGRAPHY OF ALPHA-KETO ACIDS - QUANTIFICATION OF THE BRANCHED-CHAIN ALPHA-KETO ACIDS FROM PHYSIOLOGICAL SOURCES

被引：58
作者：
CREE, TC
HUTSON, SM
HARPER, AE
机构：
[1] Department of Biochemistry, University of Wisconsin-Madison, Madison
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 01期
关键词：
D O I：
10.1016/0003-2697(79)90638-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Gas-liquid chromatography of several α-keto acids as their O-trimethylsilyl-quinoxalinol derivatives is described. Application of the method to quantification of branched-chain α-keto acids from several physiological sources is emphasized. The α-keto acids are partially purified by deproteinization with acetone, cation-exchange chromatography on Dowex 50W-X8 (H+), and conversion to quinoxalinol derivatives by reaction with o-phenylenediamine in acid solution. Prior to gas-liquid chromatography, the quinoxalinols are trimethylsilylated with N,O-bis(trimethylsilyl)trifluoroacetamide in pyridine. © 1979.
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页码：156 / 163
页数：8
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