INHIBITION OF K+ FLUX INTO RAT-LIVER MITOCHONDRIA BY DICYCLOHEXYLCARBODIIMIDE

被引:42
作者
GAUTHIER, LM
DIWAN, JJ
机构
[1] Biology Department, Rensselaer Polytechnic Institute Troy
关键词
D O I
10.1016/S0006-291X(79)80017-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dicyclohexylcarbodiimide (DCCD), which has been reported to react covalently with a subunit of the ATP synthase complex involved in proton translocation, decreases the rate of unidirectional K+ flux into rat liver mitochondria in the presence of the respiratory substrate, succinate. In contrast, oligomycin does not affect the rate of K+ influx. At concentrations which completely block ADP-stimulated respiration, DCCD increases the apparent Km for K+ of the K+ transport mechanism, while having little effect on the Vmax of K+ influx. Inhibition of K+ influx by DCCD is observed over the whole pH range tested from 6.8 to 8.0. A slight, but reproducible, decrease in the pH-dependence of K+ influx is observed in the presence of DCCD. © 1979 Academic Press, Inc. All rights of reproduction in any form reserved.
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页码:1072 / 1079
页数:8
相关论文
共 24 条
[1]   DICYCLOHEXYLCARBODIIMIDE - AN INHIBITOR OF OXIDATIVE PHOSPHORYLATION [J].
BEECHEY, RB ;
HOLLOWAY, CT ;
KNIGHT, IG ;
ROBERTON, AM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1966, 23 (01) :75-&
[2]   IDENTIFICATION OF SITE OF ACTION OF NN'-DICYCLOHEXYLCARBONDI-IMIDE AS A PROTEOLIPID IN MITOCHONDRIAL MEMBRANES [J].
CATTELL, KJ ;
LINDOP, CR ;
KNIGHT, IG ;
BEECHEY, RB .
BIOCHEMICAL JOURNAL, 1971, 125 (01) :169-&
[3]   ENERGY-DEPENDENT EXCHANGE OF K+ IN HEART-MITOCHONDRIA - K+ EFFLUX [J].
CHAVEZ, E ;
JUNG, DW ;
BRIERLEY, GP .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1977, 183 (02) :460-470
[4]   OLIGOMYCIN-DEPENDENT IONOPHORIC PROTEIN SUBUNIT OF MITOCHONDRIAL ADENOSINE-TRIPHOSPHATASE [J].
CRIDDLE, RS ;
PACKER, L ;
SHIEH, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (10) :4306-4310
[5]  
DECKER SJ, 1978, J BIOL CHEM, V253, P6738
[6]   DICYCLOHEXYLCARBODIIMIDE-BINDING PROTEIN OF RAT-LIVER MITOCHONDRIA AS A PRODUCT OF MITOCHONDRIAL PROTEIN-SYNTHESIS [J].
DIANOUX, AC ;
BOF, M ;
VIGNAIS, PV .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 88 (01) :69-77
[7]  
DIWAN JJ, 1977, INDIAN J BIOCHEM BIO, V14, P342
[8]   K+ FLUXES AND MITOCHONDRIAL-MEMBRANE POTENTIAL [J].
DIWAN, JJ ;
TEDESCHI, H .
FEBS LETTERS, 1975, 60 (01) :176-179
[9]   EFFECTS ON MITOCHONDRIAL K-FLUX OF PH, K-CONCENTRATION, AND N-ETHYL MALEIMIDE [J].
DIWAN, JJ ;
LEHRER, PH .
MEMBRANE BIOCHEMISTRY, 1978, 1 (1-2) :43-60
[10]  
DIWAN JJ, 1979, BIOPH SOC ABSTR