IMMUNOFLUORESCENT DETECTION OF BOVINE PAPILLOMAVIRUS E4 ANTIGEN IN THE CYTOPLASM OF CELLS PERMISSIVE INVITRO FOR VIRAL-DNA AMPLIFICATION

被引:16
作者
JAREBORG, N [1 ]
BURNETT, S [1 ]
机构
[1] BIOMED CTR,DEPT MED GENET,S-75123 UPPSALA,SWEDEN
关键词
D O I
10.1099/0022-1317-72-9-2269
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The E4 gene of several human papillomavirus types is expressed in association with vegetative viral DNA synthesis in differentiated epidermal cells. To develop reagents to study expression of the bovine papillomavirus type 1 (BPV-1) E4 gene in warts and in virus-transformed cell lines, rabbit polyclonal antiserum was raised to the BPV-1 E4 antigen produced as a fusion polypeptide in Escherichia coli. By immunoblotting analysis of productively infected bovine fibropapilloma tissue, E4-related proteins of 16K, 21K, 30K and 42K were detected. In some but not all C127 cell lines transformed by BPV-1 or by a replication-competent BPV-1 deletion mutant, cytoplasmic E4 antigen with a predominantly perinuclear localization was detected by immunofluorescence analysis in a subpopulation of cells in stationary-phase cultures. The E4-expressing cells were identified by their grossly enlarged size to represent the same cell subpopulation shown earlier to support BPV-1 DNA amplification. The observation of synthesis of the E4 protein in association with viral DNA amplification in this system provides further evidence that there is a switch in viral early region gene expression in a subpopulation of division-arrested cells, which may accurately reflect events occurring during the vegetative phase of BPV-1 replication in terminally differentiated cells in vivo.
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页码:2269 / 2274
页数:6
相关论文
共 14 条
  • [1] IDENTIFICATION OF THE PROTEIN ENCODED BY THE E6 TRANSFORMING GENE OF BOVINE PAPILLOMAVIRUS
    ANDROPHY, EJ
    SCHILLER, JT
    LOWY, DR
    [J]. SCIENCE, 1985, 230 (4724) : 442 - 445
  • [2] DIFFERENTIAL PROMOTER UTILIZATION BY THE BOVINE PAPILLOMAVIRUS IN TRANSFORMED-CELLS AND PRODUCTIVELY INFECTED WART TISSUES
    BAKER, CC
    HOWLEY, PM
    [J]. EMBO JOURNAL, 1987, 6 (04) : 1027 - 1035
  • [3] BREITBURD F, 1987, CANCER CELL, V5, P115
  • [4] LOSS OF BOVINE PAPILLOMAVIRUS DNA-REPLICATION CONTROL IN GROWTH-ARRESTED TRANSFORMED-CELLS
    BURNETT, S
    KIESSLING, U
    PETTERSSON, U
    [J]. JOURNAL OF VIROLOGY, 1989, 63 (05) : 2215 - 2225
  • [5] INDUCTION OF BOVINE PAPILLOMAVIRUS-E2 GENE-EXPRESSION AND EARLY REGION TRANSCRIPTION BY CELL-GROWTH ARREST - CORRELATION WITH VIRAL-DNA AMPLIFICATION AND EVIDENCE FOR DIFFERENTIAL PROMOTER INDUCTION
    BURNETT, S
    STROM, AC
    JAREBORG, N
    ALDERBORN, A
    DILLNER, J
    MORENOLOPEZ, J
    PETTERSSON, U
    KIESSLING, U
    [J]. JOURNAL OF VIROLOGY, 1990, 64 (11) : 5529 - 5541
  • [6] A NOVEL SPONTANEOUS MUTATION OF THE BOVINE PAPILLOMAVIRUS-1 GENOME
    BURNETT, S
    MORENOLOPEZ, J
    PETTERSSON, U
    [J]. PLASMID, 1988, 20 (01) : 61 - 74
  • [7] BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5
  • [8] SEQUENCE DIVERGENCE YET CONSERVED PHYSICAL CHARACTERISTICS AMONG THE E4 PROTEINS OF CUTANEOUS HUMAN PAPILLOMAVIRUSES
    DOORBAR, J
    CONERON, I
    GALLIMORE, PH
    [J]. VIROLOGY, 1989, 172 (01) : 51 - 62
  • [9] IDENTIFICATION OF THE HUMAN PAPILLOMA VIRUS-1A E4 GENE-PRODUCTS
    DOORBAR, J
    CAMPBELL, D
    GRAND, RJA
    GALLIMORE, PH
    [J]. EMBO JOURNAL, 1986, 5 (02) : 355 - 362
  • [10] HARLOW E, 1988, ANTIBODIES LABORATOR, P505