CLONING OF A CDNA-ENCODING A NOVEL PUTATIVE G-PROTEIN-COUPLED RECEPTOR EXPRESSED IN SPECIFIC RAT-BRAIN REGIONS

被引:66
作者
MEYERHOF, W
PAUST, HJ
SCHONROCK, C
RICHTER, D
机构
[1] Institut für Zellbiochemie und klinische Neurobiologie UKE, D-2000 Hamburg 20, FRG
关键词
D O I
10.1089/dna.1991.10.689
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA clone encoding a novel putative G-protein-coupled receptor was isolated from a rat brain cDNA library using a PCR-amplified cDNA fragment as a hybridization probe. The 3,615-bp-long nucleotide sequence predicts a single open reading frame of 1,173 bp coding for 391 amino acids, giving a calculated molecular weight of 42.75 kD. The amino acid sequence shares features common to many other receptors, including the seven membrane-spanning hydrophobic regions and putative asparagine-linked glycosylation and phosphorylation sites. Northern blot analysis reveals that a corresponding approximately 3.7-kb mRNA is expressed in specific brain regions such as hypothalamus, cortex, hippocampus, and thalamus but not in other organs analyzed. Although the ligand for this receptor has not yet been identified, it shares some simularities with the vascular type-1 angiotensin II receptor, the vasoactive intestinal peptide (VIP) receptor, and the chemotactic receptors for human C5a anaphylatoxin and the formyl peptide fMet-Leu-Phe.
引用
收藏
页码:689 / 694
页数:6
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共 16 条
  • [1] THE HUMAN N-FORMYLPEPTIDE RECEPTOR - CHARACTERIZATION OF 2 CDNA ISOLATES AND EVIDENCE FOR A NEW SUBFAMILY OF G-PROTEIN-COUPLED RECEPTORS
    BOULAY, F
    TARDIF, M
    BROUCHON, L
    VIGNAIS, P
    [J]. BIOCHEMISTRY, 1990, 29 (50) : 11123 - 11133
  • [2] ROLE OF EXTRACELLULAR DISULFIDE-BONDED CYSTEINES IN THE LIGAND-BINDING FUNCTION OF THE BETA-2-ADRENERGIC RECEPTOR
    DOHLMAN, HG
    CARON, MG
    DEBLASI, A
    FRIELLE, T
    LEFKOWITZ, RJ
    [J]. BIOCHEMISTRY, 1990, 29 (09) : 2335 - 2342
  • [3] MOLECULAR-CLONING OF A GENE ENCODING THE HISTAMINE-H2-RECEPTOR
    GANTZ, I
    SCHAFFER, M
    DELVALLE, J
    LOGSDON, C
    CAMPBELL, V
    UHLER, M
    YAMADA, T
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (02) : 429 - 433
  • [4] THE CHEMOTACTIC RECEPTOR FOR HUMAN-C5A ANAPHYLATOXIN
    GERARD, NP
    GERARD, C
    [J]. NATURE, 1991, 349 (6310) : 614 - 617
  • [5] PROTEIN-KINASE RECOGNITION SEQUENCE MOTIFS
    KEMP, BE
    PEARSON, RB
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (09) : 342 - 346
  • [7] A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN
    KYTE, J
    DOOLITTLE, RF
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) : 105 - 132
  • [8] SELECTIVE AMPLIFICATION AND CLONING OF 4 NEW MEMBERS OF THE G-PROTEIN COUPLED RECEPTOR FAMILY
    LIBERT, F
    PARMENTIER, M
    LEFORT, A
    DINSART, C
    VANSANDE, J
    MAENHAUT, C
    SIMONS, MJ
    DUMONT, JE
    VASSART, G
    [J]. SCIENCE, 1989, 244 (4904) : 569 - 572
  • [9] MOLECULAR-CLONING OF A NOVEL PUTATIVE G-PROTEIN COUPLED RECEPTOR EXPRESSED DURING RAT SPERMIOGENESIS
    MEYERHOF, W
    MULLERBRECHLIN, R
    RICHTER, D
    [J]. FEBS LETTERS, 1991, 284 (02) : 155 - 160
  • [10] ISOLATION OF A CDNA-ENCODING THE VASCULAR TYPE-1 ANGIOTENSIN-II RECEPTOR
    MURPHY, TJ
    ALEXANDER, RW
    GRIENDLING, KK
    RUNGE, MS
    BERNSTEIN, KE
    [J]. NATURE, 1991, 351 (6323) : 233 - 236