CHROMATOGRAPHIC-SEPARATION OF FACTOR-VIII ON AMINOHEXYL SEPHAROSE

Summary. A new method of factor VIII purification has been devised which involves chromatographic separation on aminohexyl‐substituted agarose. Relatively large volumes of starting material can be processed compared to the volume of agarose employed and satisfactory yields are obtained. Factor‐VIII‐clotting activity is separated from the other related substances and resultant products appear to be stable. While separation of clotting activity occurs with relative ease, the antigen and ristocetin co‐factor are hardly segregated, if at all. This provides a little more information about the interrelation of these substances. Results suggest that ion‐exchange is involved in the mechanism of separation but additional hydrophobic or steric effects cannot be ruled out. Copyright © 1979, Wiley Blackwell. All rights reserved