MOLECULAR CHARACTERIZATION AND GENETIC-ORIGIN OF THE BRASSICA-NAPUS ACETOHYDROXYACID SYNTHASE MULTIGENE FAMILY

被引：81

作者：

RUTLEDGE, RG ^{[1
]}

QUELLET, T ^{[1
]}

HATTORI, J ^{[1
]}

MIKI, BL ^{[1
]}

机构：

[1] AGR CANADA,PLANT RES CTR,OTTAWA K1A 0C6,ONTARIO,CANADA

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 229卷 / 01期

关键词：

BRASSICA; ACETOHYDROXYACID SYNTHASE; GENETIC ORIGINS; GENE FAMILY; DNA SEQUENCE;

D O I：

10.1007/BF00264210

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Brassica napus rapeseed cultivar Topas contains an acetohydroxyacid synthase (AHAS) multigene family consisting of five members (AHAS 1-5). DNA sequence analysis indicate that AHAS1 and AHAS3 share extensive homology. They probably encode the AHAS enzymes essential for plant growth and development. AHAS2 has diverged significantly from AHAS1 and AHAS3 and has unique features in the coding region of the mature polypeptide, transit peptide and upstream non-coding DNA, which raises the possibility that it has a distinct function. AHAS4 and AHAS5 have interrupted coding regions and may be defective. The complexity of the AHAS multigene family in the allotetraploid species B. napus is much greater than reported for Arabidopsis thaliana and Nicotiana tabacum. Analysis of the presumptive progenitor diploid species B. campestris and B. oleracea indicated that AHAS2, AHAS3 and AHAS4 originate from the A genome, whereas AHAS1 and AHAS5 originate from the C genome. Further variation within each of the AHAS genes in these species was found.

引用

页码：31 / 40

页数：10

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