CHEMICALLY DEFINED MEDIUM FOR THE PRODUCTION OF BIOLOGICALLY-ACTIVE SUBSTANCES OF CHO CELLS

被引：9

作者：

HATA, J

TAMURA, T

YOKOSHIMA, S

YAMASHITA, S

KABENO, S

MATSUMOTO, K

ONODERA, K

机构：

[1] NISSUI PHARMACEUT CO LTD,RES LAB,YUUKI,IBARAKI 307,JAPAN

[2] UNIV TOKYO,DEPT AGR CHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

CYTOTECHNOLOGY | 1992年 / 10卷 / 01期

关键词：

RECOMBINANT CHO; HGH; DHFR; MTX; CHEMICALLY DEFINED MEDIUM;

D O I：

10.1007/BF00376095

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant CHO cell line (GT19) secreting a high level of human growth hormone (hGH) was constructed with amplification of the introduced hGH gene. The cells grew well in the alpha MEM medium supplemented with 5% dialyzed fetal calf serum (dFCS), but not with less than 1% dFCS. Therefore we examined various medium components and obtained an improved medium which supported cell growth at low serum concentrations. The production of hGH by the cells was also enhanced in this medium.

引用

页码：9 / 14

页数：6

共 15 条

[1]

AYUSAWA D, 1981, J BIOL CHEM, V256, P2005

[2] HIGH EXPRESSION IN MAMMALIAN-CELLS WITHOUT AMPLIFICATION [J].