MAGNETIC-RESONANCE SPECTROSCOPY DETECTS METABOLIC DIFFERENCES BETWEEN 7 DUNNING RAT PROSTATE TUMOR SUBLINES WITH DIFFERENT BIOLOGICAL BEHAVIOR

In this study, it was investigated whether prostate tumor biological parameters correlate with metabolic profiles. H-1 and P-31 magnetic resonance spectra were acquired from perchloric acid extracts of seven Dunning R-3327 prostate tumor sublines. Several metabolic ratios, for example, phosphocholine/total phosphate, choline/total creatine, and inositol/total creatine, did not correlate specifically with one biological characteristic-but, based on each of these ratios, the well-differentiated, nonmetastatic, and hormone-dependent sublines could be discriminated from the poorly differentiated or anaplastic, metastatic, and hormone-independent sublines. The glycerophosphoethanolamine/total phosphate, glycerophosphocholine/total phosphate, and phosphocreatine/total phosphate ratios correlated with differentiation grade, and the differences in glycerophosphorylglycerol/total phosphate ratio between metastatic and nonmetastatic sublines was highly significant. No correlation for hormonal sensitivity with any of the metabolites measured could be found, neither by P-31 nor by H-1 MRS. (C) 1994 Wiley-Liss, Inc.