DETECTION OF MINIMAL AMOUNTS OF DNA BY ELECTRON-MICROSCOPY USING SIMPLIFIED SPREADING PROCEDURES

被引：8

作者：

BOCK, CT ^{[1
]}

ZENTGRAF, H ^{[1
]}

机构：

[1] DEUTSCH KREBSFORSCHUNGSZENTRUM,NEUENHEIMER FELD 242,W-6900 HEIDELBERG,GERMANY

来源：

CHROMOSOMA | 1993年 / 102卷 / 04期

关键词：

D O I：

10.1007/BF00352398

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron microscopic examination of nucleic acids requires the use of special spreading techniques. The classical method was developed by Kleinschmidt and Zahn in 1959. Modifications of this method increased sensitivity to allow detection of a total amount of about 1 x 10(-3) mug of single-stranded DNA and 2 x 10(-5) mug of double-stranded DNA. Here we describe two rapid and simple procedures increasing sensitivity by 1-2 orders of magnitude to visualize at least 1 x 10(-5) mug of single- and/or double-stranded DNA.

引用

页码：249 / 252

页数：4

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