USE OF THIOPHILIC ADSORPTION IN THE PURIFICATION OF BIOTINYLATED FAB FRAGMENTS

被引：12

作者：

LUTOMSKI, D

JOUBERTCARON, R

BOURIN, P

BLADIER, D

CARON, M

机构：

[1] UNIV PARIS NORD,UFR LEONARD DE VINCI,BIOCHIM & TECHNOL PROT LAB,F-93012 BOBIGNY,FRANCE

[2] HOP PERCY,CTR TRANSFUS SANGUINE ARMEES,IMMUNOL CELLULAIRE LAB,F-92141 CLAMART,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 664卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00399-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the purification and biotinylation of Fab fragments, using thiophilic adsorption (T-gel), is described. The T-gel was used to purify an IgG fraction directly in the buffer suitable for biotinylation, and to adsorb intact IgGs and papain after enzymatic digestion. For the final step, Fc fragments were removed with a protein A column.

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页码：79 / 82

页数：4

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