TRANSGLUTAMINASE ACTIVITY IN RABBIT ARTICULAR CHONDROCYTES IN CULTURE

被引:20
作者
DEMIGNOT, S
BORGE, L
ADOLPHE, M
机构
[1] Laboratoire de Pharmacologie Cellulaire, Ecole Pratique des Hautes Etudes, Centre de Recherches Biomédicales des Cordeliers, Paris, 75006
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1995年 / 1266卷 / 02期
关键词
ARTICULAR CHONDROCYTE; TRANSGLUTAMINASE ACTIVITY; RETINOIC ACID; COLLAGEN SYNTHESIS; AGING; DIFFERENTIATION;
D O I
10.1016/0167-4889(95)00013-I
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In vivo, articular chondrocytes produce an important amount of extracellular matrix (cartilage) whose quality is impaired upon inflammation or aging leading to arthritis or arthrosis. Transglutaminases (EC 2.3.2.13) are a family of enzymes which have been shown to be involved in extracellular matrix stabilization, cell differentiation and possibly in initiation and propagation of inflammatory diseases. It is therefore of interest to study transglutaminase activity in chondrocytes. Transglutaminase activity was studied in rabbit articular chondrocytes in primary culture, where cells are in a well-differentiated state as assessed by collagen-type synthesis, as well as in subculture and in retinoic acid-treated cells, where cells are in a dedifferentiated state. Results showed that two different TGases activities are expressed in chondrocytes. One, down-regulated upon retinoic acid treatment of cells, preferentially membrane bound and strongly activated upon trypsin treatment of cell lysates, is expressed at a high level in primary culture. The other one is up-regulated upon retinoic acid treatment, preferentially cytosolic and inactivated upon trypsin treatment of cell lysates. The rate of expression of the TGase down-regulated by RA seems to correlate with the differentiation state of the chondrocyte. This suggests that this TGase activity may have a physiological role in cartilage and merits further study.
引用
收藏
页码:163 / 170
页数:8
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