EFFECT OF HEMATOPOIETIC GROWTH-FACTORS ON THE PROLIFERATION OF ACUTE MYELOID AND LYMPHOID LEUKEMIAS

被引：6

作者：

AGLIETTA, M

DEFELICE, L

STACCHINI, A

SANAVIO, F

SEVERINO, A

SIMONE, F

PIACIBELLO, W

MANDELLI, F

机构：

[1] Clinica Medica A, Department of Biomedical Science and Human Oncology, University of Turin, Rome

[2] Division of Hematology, Department of Human Biopathology, University La Sapienza, Rome

来源：

LEUKEMIA & LYMPHOMA | 1990年 / 2卷 / 3-4期

关键词：

Cell kinetics; Differentiation; Growth factors; In vitro; Leukemia;

D O I：

10.3109/10428199009053525

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The effect of G-CSF, GM-CSF, IL-1 and IL-3 on the proliferation of acute leukemia cells (evaluated as 3HTdR uptake) was investigated in short-term liquid cultures and compared with that observed on normal bone marrow (BM) populations enriched for immature cells. In acute myeloid leukemias (AML), a marked leukemic proliferation was induced in 10/18 cases by IL-3, in 9/18 by GM-CSF, in 7/18 by IL-1 and in 4/18 by G-CSF. In acute lymphoid leukemias (ALL), marked stimulation was observed in 7/11 cases with IL-3 and in 5/11 with GM-CSF, whereas IL-1 and G-CSF were ineffective. Both in AML and ALL, the combination of several factors did not result in an additive synergistic effect. Purified normal BM cells responded to all four growth factors and their combinations produced an additive effect on cell proliferation which probably relates to the heterogeneity of the cell populations studied. The effect of a G-CSF, GM-CSF, IL-1 and IL-3 on the proliferation of acute leukemia cells by different growth factors suggests that caution should be exercised in their clinical use in these diseases © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：207 / 214

页数：8

共 37 条

[1]

Metcalf D., The granulocyte-macrophage colony stimulating factors, Science, 229, pp. 16-22, (1985)

[2]

Clark S.C., Kamen R., The human hematopoietic colony-stimulating factor, Science, 79, pp. 1229-1237, (1987)

[3]

Sieff C.A., Hematopoietic growth factor, J. Clin. Invest, 236, pp. 1549-1555, (1987)

[4]

Delwel R., Salem M., Pellens C., Dorssers L., Wagemaker G., Clark S., Lovemberg B., Growth regulation of human acute myeloid leukemia: effects of five recombinant hematopoietic factors in a serum-free culture system, Blood, 72, pp. 1944-1949, (1988)

[5]

Delwel R., Dorssers L., Touw I., Wagemaker G., Lowemberg B., Human recombinant multilineages colony stimulating factor (interleukin 3) stimulator of acute myelocitic leukemia progenitor cells in vitro, Blood, 70, pp. 333-336, (1987)

[6]

Saeland S., Caux C., Favre G., Aubry J.P., Mannoni P., Pebusque J.M., Gentilhomme O., Otsuka T., Yokota T., Arai N., Arai K., Banchereau J., De Vries J.E., Effect of recombinant human interleukin 3 on CD34 enriched normal hematopoietic progenitors and on myeloblasts leukemia cells, Blood, 72, pp. 1580-1588, (1988)

[7]

Lowemberg B., Salem M., Delwel R., Effect of recombinant multi CSF, GM-CSF, G-CSF and M-CSF on the proliferation and maturation of human AML in vitro, Blood Cells, 14, pp. 539-549, (1988)

[8]

Vellenga E., Young D.C., Wagner K., Wiper D., Ostapovicz D., Griffin J.D., The effects of GM-CSF and G-CSF in promoting growth of clonogenic cells in acute myeloblastic leukemia, Blood, 69, pp. 1771-1776, (1987)

[9]

Begley C.G., Metcalf D., Nichola N.A., Primary human myeloid leukemia cells: comparative responsiveness to proliferative stimulation by GM-CSF or G-CSF and membrane expression of CSF receptors, Leukemia, 1, pp. 1-8, (1987)

[10]

Kelleher C., Miyauchi J., Wong G., Clark S., Minden M.D., McCulloch E.A., Synergism between recombinant growth factors, GM-CSF and G-CSF, acting on the blast cells of acute myeloblastic leukemia, Blood, 69, pp. 1498-1503, (1987)

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