PROLIFERATIVE RESPONSE OF TAX1-TRANSDUCED PRIMARY HUMAN T-CELLS TO ANTI-CD3 ANTIBODY STIMULATION BY AN INTERLEUKIN-2-INDEPENDENT PATHWAY

被引：104

作者：

AKAGI, T ^{[1
]}

SHIMOTOHNO, K ^{[1
]}

机构：

[1] NATL CANC CTR,RES INST,DIV VIROL,5-1-1 TSUKIJI,CHUO KU,TOKYO 104,JAPAN

来源：

JOURNAL OF VIROLOGY | 1993年 / 67卷 / 03期

关键词：

D O I：

10.1128/JVI.67.3.1211-1217.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The growth properties of human T-cell leukemia virus Tax1-transduced primary human T cells derived from peripheral blood lymphocytes were compared with those of the same subset of T cells transduced with a control vector. Tax1-transduced T cells exhibited slightly elevated responsiveness to externally added interleukin-2 (IL-2) and a markedly higher proliferative response to stimulation with anti-CD3 antibody. The proliferation after anti-CD3 antibody stimulation was mainly via an IL-2-independent pathway. Therefore, some other mechanism than the previously proposed IL-2 autocrine model seems to be involved in the process of deregulation of T-cell proliferation by Tax1. Moreover, Tax1-transduced T cells have continued to proliferate in medium containing IL-2 long after control T cells ceased to grow, and so they are considered to be immortalized.

引用

页码：1211 / 1217

页数：7

共 58 条

[1]

AKAGI T, 1991, GENE, V106, P255, DOI 10.1016/0378-1119(91)90207-R

[2]

ARIMA N, 1986, BLOOD, V68, P779

[3]

CANN AJ, 1988, ONCOGENE, V3, P123

[4] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[5] REGULATION OF THE HUMAN INTERLEUKIN-2 RECEPTOR ALPHA-CHAIN PROMOTER - ACTIVATION OF A NONFUNCTIONAL PROMOTER BY THE TRANSACTIVATOR GENE OF HTLV-1 [J].

CROSS, SL ;

FEINBERG, MB ;

WOLF, JB ;

HOLBROOK, NJ ;

WONGSTAAL, F ;

LEONARD, WJ .

CELL, 1987, 49 (01) :47-56

[6] THE PX PROTEIN OF HTLV-I IS A TRANSCRIPTIONAL ACTIVATOR OF ITS LONG TERMINAL REPEATS [J].

FELBER, BK ;

PASKALIS, H ;

KLEINMANEWING, C ;

WONGSTAAL, F ;

PAVLAKIS, GN .

SCIENCE, 1985, 229 (4714) :675-679

[7] POWERFUL AND VERSATILE ENHANCER-PROMOTER UNIT FOR MAMMALIAN EXPRESSION VECTORS [J].

FOECKING, MK ;

HOFSTETTER, H .

GENE, 1986, 45 (01) :101-105

[8] C-FOS PROMOTER TRANS-ACTIVATION BY THE TAX1 PROTEIN OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I [J].

FUJII, M ;

SASSONECORSI, P ;

VERMA, IM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (22) :8526-8530

[9]

FUJII M, 1991, ONCOGENE, V6, P1023

[10]

GEPPERT TD, 1987, J IMMUNOL, V138, P1660

← 1 2 3 4 5 6 →