PROBING THE S-1' SUBSITE SELECTIVITY OF AN INDUSTRIAL ALKALINE PROTEASE IN ANHYDROUS T-BUTANOL

Study of the selectivity of s-1'-subsite of alcalase catalyzed reaction in t-butanol shows that the rate of product formation depends on the size of the nucleophiles. D- and L-amino acids as nucleophile form the product with the same reaction rate. Interpretation of this selectivity by molecular modeling using crystal structure of subtilisin Carlsberg complexed with it's substrate gives clear evidence.